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. 2018 Jan 8;5(3):399–413. doi: 10.1016/j.jcmgh.2018.01.003

Figure 2.

Figure 2

HFD-plus-one binge ethanol challenge induces liver fibrosis. Mice were subject to chow diet plus maltose gavage (chow), acute ethanol binge (5 g/kg, 1B), high-fat diet plus maltose gavage (HFD), or HFD-plus-ethanol binge (HFD+1B). Mice were euthanized 9 hours after gavage, and then liver samples were collected and analyzed. (A) Representative images of H&E staining, Sirius Red and Masson Trichrome staining of liver tissues. (B) Sirius Red–positive area was quantified, and body weight was measured. (C) Liver tissues were collected for quantitative RT-PCR analyses of fibrogenic genes. N = 6–7 in each group. *P < .05, **P < .01, ***P < .001.