Figure 13.

TBE-31 suppresses oxidative stress in HFFr-fed Nrf2^+/+mice. The abundance of oxidative stress-associated biomarkers and expression of antioxidant Nrf2-target genes examined in livers from Study 1. (A) Malondialdehyde levels. (B) Oxidized protein levels shown as a representative Oxyblot, with densitometric quantification below. Lanes 1, 3, 5, and 7 negative controls; 2, RC-fed DMSO; 4, RC-fed TBE-31; 6, HFFr-fed DMSO; 8, HFFr-fed TBE-31. (C) Ratio of GSH to GSSG. (D) qRT-PCR for Gclc, Gclm, Gpx2, Nqo1, Gstm1, Hmox1, Txn1, Txnrd1, Slc7a11, Catalase (Cat), and Prdx6. In all cases, white bars represent DMSO and black bars represent TBE-31. In A, C, and D, n = 8–12. In B, n = 6. Data are means ± SEM. Significant differences are represented as: ^∗,$P < .05; ^∗∗,$$P < .01; ^{∗∗∗,$$$}P < .001.