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. 2017 Dec 13;5(3):367–398. doi: 10.1016/j.jcmgh.2017.11.016

Figure 17.

Figure 17

TBE-31 does not decrease expression of lipogenic transcription factors or fatty acid synthesis enzymes in livers of HFFr-fed Nrf2-/-mice. The expression of transcription factors associated with lipid metabolism, and their target genes, were examined in livers from Nrf2+/+ and Nrf2-/- mice that had been fed either a RC or HF30Fr (HFFr) diet and treated with DMSO or TBE-31. (A) qRT-PCR for the lipogenic transcription factors Srebf1, Mlxipl, Lxrα and Xbp1s. (B) Representative Srebp-1c immunoblots of cytoplasmic (cSrebp-1c) and nuclear (nSrebp-1c) protein on left side, with plots of densitometric scans on right side (n = 4 biologic replicates). (C) qRT-PCR for the fatty acid synthesis genes Acaca, Acly, Fasn, Scd1, and Dgat2. (D) qRT-PCR for the lipid import gene Cd36 and the export genes Mttp and ApoB. White bars, DMSO treated; black bars, TBE-31 treated. In A, C, and D, 6–8 mice per group. In B, n = 4. Results are means ± SEM. Significant increases relative to that found in livers of RC-fed DMSO-treated Nrf2+/+ mice are indicated by: *P < .05; **P < .01; ***P < .001. Significant decreases relative to HF30Fr-fed DMSO-treated Nrf2+/+ mice are indicated: $P < .05; $$P < .01.