Table 1.
qRT-PCR Primers and Probe Sets
| Gene | Protein encoded | Assay ID |
|---|---|---|
| Acaca | Acetyl-CoA carboxylase 1 | Mm01304257_m1 |
| Acly | ATP Citrate Lyase | Mm01302282_m1 |
| Acot7 | Acyl-CoA Thioesterase 7 | Mm00460107_m1 |
| Acox2 | Acyl-CoA Oxidase 2 | Mm00446408_m1 |
| ApoB | Apolipoprotein B | Mm01545150_m1 |
| Adrp | Adipose differentiation related protein | Mm00475794_m1 |
| Atf4 | Activating Transcription Factor 4 | Mm00515325_g1 |
| ɑSma | actin, alpha 2, smooth muscle, aorta | Mm00725412_s1 |
| Bcl2 | B cell leukemia/lymphoma 2 | Mm00477631_m1 |
| Cat | Catalse | Mm00437992_m1 |
| Cd36 | Cluster of differentiation 36 | Mm01135198_m1 |
| Ces1g | Carboxylesterase 1 g | Mm00491334_m1 |
| Chop | CCAAT/enhancer-binding protein homologous protein | Mm01135937_g1 |
| Col1a1 | Collagen type I alpha 1 chain | Mm00801666_g1 |
| Cox2 | Cyclooxygenase-2 | Mm03294838_g1 |
| Cpt1a | Carnitine palmitoyltransferase 1A | Mm01231183_m1 |
| Dgat1 | Diacylglycerol O-acyltransferase 1 | Mm00515643_m1 |
| Dgat2 | Diacylglycerol O-acyltransferase 2 | Mm00499536_m1 |
| Elastase | Elastase | Mm00712898_m1 |
| Fasn | Fatty acid synthase | Mm00662319_m1 |
| Gclc | Glutamate-cysteine ligase catalytic subunit | Mm00802655_m1 |
| Gclm | Glutamate-cysteine ligase modifier subunit | Mm01324400_m1 |
| Gpx2 | Glutathione peroxidase 2 | Mm00850074_g1 |
| Gsta4 | Glutathione S-transferase alpha 4 | Mm00494803_m1 |
| Gstm1 | Glutathione S-transferase mu 1 | Mm00833915_g1 |
| Hmox1 | Heme oxygenase 1 | Mm00516005_m1 |
| Ifng | Interferon gamma | Mm01168134_m1 |
| Il1b | Interleukin 1 beta | Mm00434228_m1 |
| Lipin1 | Lipin 1 | Mm00550511_m1 |
| Lxrɑ | Nuclear receptor subfamily 1 group H member 3 | Mm00443451_m1 |
| Mcp1 | Monocyte chemotactic protein 1 | Mm00441242_m1 |
| Mgpat | 1-acylglycerol-3-phosphate O-acyltransferase 9 | Mm04211965_m1 |
| Mixipl | MLX interacting protein-like (also known as Chrebp) | Mm02342723_m1 |
| Mmp9 | Matrix metallopeptidase 9 | Mm00442991_m1 |
| Mpo | Myeloperoxidase | Mm01298424_m1 |
| Mttp | Microsomal triglyceride transfer protein | Mm01321412_g1 |
| Nos2 | Nitric oxide synthase 2 | Mm00440495_g1 |
| Nqo1 | NAD(P)H:quinone oxidoreductase 1 | Mm01253561_m1 |
| Perk | Eukaryotic translation initiation factor 2 alpha kinase 3 | Mm00438700_m1 |
| Pparɑ | Peroxisome proliferator-activated receptor alpha | Mm00440939_m1 |
| Prdx6 | Peroxiredoxin 6 | Mm00725435_s1 |
| Ptgr1 | Prostaglandin reductase 1 | Mm00482476_m1 |
| Scad | Short-chain acyl-CoA dehydrogenase | Mm00431617_m1 |
| Scd1 | Stearoyl-Coenzyme A desaturase 1 | Mm00772290_m1 |
| Slc7a11 | Solute carrier family 7 member 11 | Mm01292536_m1 |
| Srebf1 | Sterol regulatory element binding transcription factor 1 | Mm00550338_m1 |
| Tgfb | Transforming growth factor beta 1 | Mm01178820_m1 |
| Tnfɑ | Tumor necrosis factor alpha | Mm00443258_m1 |
| Txn1 | Thioredoxin 1 | Mm00726847_s1 |
| Txnrd1 | Thioredoxin reductase 1 | Mm00443675_m1 |
qRT-PCR, quantitative reverse transcriptase polymerase chain reaction.