Table 2.
TBE-31 Decreases Histological Features Associated With NASH and Cirrhosis in Livers of Mice Fed a HFFr Diet
| Parameter | Value (n) |
|
|---|---|---|
| DMSO | TBE-31 | |
| NASa (maximum 8) | ||
| RC | 0.091 (11) | 0.182 (11) |
| HFFr | 4.901 (8) | 2.917 (8) |
| Steatosis component of NASb (0–3) | ||
| RC | 0.0 (11) | 0.0 (11) |
| HFFr | 2.900 (8) | 1.833 (8) |
| Inflammatory component of NASc (0–3) | ||
| RC | 0.091 (11) | 0.182 (11) |
| HFFr | 1.300 (8) | 1.000 (8) |
| Ballooning component of NASd (0–2) | ||
| RC | 0.0 (11) | 0.0 (11) |
| HFFr | 0.700 (8) | 0.0833 (8) |
| Fibrosis stagee (0–3) | ||
| RC | 0.0 (11) | 0.0 (11) |
| HFFr | 0.727 (8) | 0.167 (8) |
The NAS-based evaluation of severity of NAFLD was found to be significantly higher in livers of HFFr-fed mice than in livers of RC-fed animals (Kruskal-Wallis test; P < .0001). Livers of mice fed the HFFr diet and treated with DMSO vehicle control had significantly higher NAS than those of HFFr-fed mice treated with TBE-31 (Kruskal-Wallis H test; P < .05).
Livers of mice fed the HFFr diet showed more steatosis than livers of their RC-fed counterparts (Kruskal-Wallis H test; P < .0001). No significant difference was observed in liver steatosis between DMSO- and TBE-31-treated mice fed on the same diet.
The inflammatory component was significantly higher in livers of mice fed on the HFFr diet when compared with their RC-fed counterparts (Kruskal-Wallis H test; P < .0001). No significant difference in liver inflammation was observed between DMSO- and TBE-31-treated mice on the same diet.
Liver ballooning was significantly higher in mice fed the HFFr diet when compared with their RC-fed counterparts (Kruskal-Wallis H test; P < .0001). Ballooning in livers of mice fed the HFFr diet and treated with DMSO was significantly higher than in livers of HFFr-fed mice treated with TBE-31 (Kruskal-Wallis H test; P < .05).
Liver fibrosis (this is not included in the NAS calculation) was significantly higher in mice fed the HFFr diet when compared with their RC-fed counterparts (Kruskal-Wallis H test; P < .0001). Fibrosis in livers of mice fed the HFFr diet and treated with DMSO was significantly higher than in livers of HFFr-fed mice treated with TBE-31 (Kruskal-Wallis H test; P < .05).