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. 2018 Feb 12;37(6):e97953. doi: 10.15252/embj.201797953

Figure EV1. Comparison of wild‐type and template‐free (TF) telomerase reconstitution systems.

Figure EV1

  1. Schematic of the 451‐nt full‐length hTR. The hTR secondary structure comprises three major structural domains: the pseudoknot (PK), conserved regions 4/5 (CR4/5), and box H/ACA domain. The template region and the two structural domains, PK, and CR4/5 (red), are minimally required for reconstituting telomerase activity in vitro.
  2. The wild‐type telomerase core enzyme comprises the full‐length hTR (green) and the catalytic TERT protein (gray). The substrate for wild‐type telomerase is a single‐stranded DNA primer (blue).
  3. TF telomerase comprises the minimally required PK and CR4/5 hTR fragments (green). The substrate for TF telomerase is duplex of a single‐stranded DNA primer (blue) pre‐annealed with an RNA template.