Skip to main content
. 2018 Feb 12;37(6):e97953. doi: 10.15252/embj.201797953

Figure 6. Telomerase utilizes deoxynucleoside diphosphates as substrate.

Figure 6

  1. Direct primer extension assays were performed with telomerase enzyme reconstituted in vivo and immuno‐purified. Telomerase was assayed in the presence of 0.165 μM 32P‐dTTP, 10 μM dTTP, 100 μM dATP as well as combinations of either 10 or 100 μM of dGMP, dGDP, or dGTP.
  2. Utilization of dGDP by RTs. Specific DNA/RNA hybrid substrates depicted harbor a template sequence for the incorporation of a single dA and a dG residue.
  3. Utilization of dGDP by DNA‐dependent DNA polymerases. The DNA/DNA hybrid substrate depicted harbors a template sequence to allow for incorporation of a single dA and a dG residue. Taq DNA polymerase (pol) and the Klenow fragment of DNA polymerases I were assayed in the presence of 0.165 μM 32P‐dATP (asterisk) and either dGMP, dGDP, or dGTP. The identity of nucleotides added are denoted to the right of the gel.