Skip to main content
. 2018 Jan 25;16(2):43. doi: 10.3390/md16020043

Figure 5.

Figure 5

BOS-102 induces ROS generation and loss of MMP in A549 cells. (A–C) A549 cells were treated with various concentrations of BOS-102 (0, 2.5, 5, 10 µM) for 48 h, the medium was discarded, and cells were incubated at 37 °C in the dark for 20 min with culture medium containing DCFDA. Cells were harvested and analysis using FACS and fluorescence microscopy (Bar = 50 µm); (D) A549 cells were treated with 5 µM BOS-102 alone or in combination with NAC (5 mM) for 48 h. ROS generation was detected by FACS after staining with DCFDA; (E,F) A549 cells were treated with various concentrations of BOS-102 (0, 2.5, 5, 10 µM) for 48 h. The cells were collected and incubated with JC-1 for 20 min at 37 °C, cells were then washed twice with PBS and the values of MMP were analyzed by FACS; (G) Immunoblotting of mito-cytochrome c and cyto-cytochrome c. The data represent mean values (±SD) obtained from three separate experiments. ** p < 0.01 vs. control group. ## p < 0.01 vs. 102(+)/NAC(−) group.