Figure 3.

HSCCC chromatogram of the crude extract from Fusarium sp. LS68 using stepwise elution with solvent systems A and B. The dotted line represents the time at which the solvent system was switched from A to B. Solvent system A: hexanes–EtOAc–CH₃OH–H₂O (6:4:5:5, v/v/v/v), solvent system B: hexanes–EtOAc–CH₃OH–H₂O (8.5:1.5:5:5, v/v/v/v); stationary phase: upper phase of solvent system A; mobile phase: lower aqueous phase of solvent system A and B; column capacity, 350 mL; rotation speed, 800 rpm; column temperature, 25 °C; flow rate, 2.0 mL/min; detection, 254 nm; sample injected, 200 mg in 6 mL biphasic solution; retention of the stationary phase, 63%; peak identification: roridin E (1), roridin E acetate (2), verrucarin L acetate (3), verrucarin J (4).