Figure 5.
Sc Ang II at 150 ng/kg/min significantly increases NCX1 protein in the aorta of rats on 2% high salt (HS), but not in rats on regular salt (RS) diet (panels A and B). Central infusion of an MR blocker (eplerenone or spironolactone), ENaC blocker (benzamil) or AT1R blocker (losartan) prevents the increase in NCX1 protein in the aorta caused by Ang II in rats on HS diet (panels C and D). Panels A and B: the summary data are for changes in NCX1 relative to the control group. A representative immunoblot shows ∼160 kDa NCX1 protein under non-reducing conditions. Values are means ± SE (n = 5–8/group). Two-way ANOVA: *P<0.05 vs. others. Panels C and D: the summary data are for changes in NCX1 protein levels relative to the vehicle; n = 6 for vehicle (Veh), n = 6 for MR blocker, n = 6 for benzamil (Ben), and n = 4 for losartan (Los). A representative immunoblot shows effect of central MR-ENaC-AT1R blockade on NCX1 protein. Student t-test: *P<0.05 vs. others