Table 1.
Effect of low-dose Ang II combined with regular or 2% high salt diet on plasma Ang II, systolic and diastolic BP and LV weight (top panel) and effects of central MR-ENaC-AT1R blockade on responses to Ang II + salt (bottom panel)
| Regular salt | High salt | Regular salt + Ang II | High salt + Ang II | |
|---|---|---|---|---|
| Plasma Ang II (pg/ml) | 14 ± 2 | 7 ± 2 | 12 ± 3 | 14 ± 2 |
| Systolic BP (mmHg) | 127 ± 1 | 131 ± 3 | 142 ± 2** | 185 ± 4* |
| Diastolic BP (mmHg) | 85 ± 2 | 87 ± 3 | 91 ± 2 | 131 ± 4* |
| LV weight (mg/100g BW) | 187 ± 9 | 204 ± 5 | 211 ± 7 | 238 ± 6* |
| High salt + Ang II | ||||
| + | + | + | + | |
| Veh (n = 11) | MR inh (n = 9) | Ben (n = 6) | Los (n = 4) | |
| Plasma Ang II (pg/ml) | 24 ± 6 | 18 ± 4 | 24 ± 2 | 14 ± 5 |
| Systolic BP (mmHg) | ||||
| Baseline | 120 ± 3 | 117 ± 1 | 116 ± 2 | 119 ± 2 |
| Average of last 3 days | 177 ± 7*,*** | 129 ± 3*** | 124 ± 2*** | 128 ± 5 |
| Diastolic BP (mmHg) | ||||
| Baseline | 88 ± 2 | 81 ± 1 | 80 ± 1 | 85 ± 3 |
| Average of last 3 days | 138 ± 8*,*** | 91 ± 2*** | 88 ± 2*** | 90 ± 5 |
| LV weight (mg/100g BW) | 262 ± 11* | 210 ± 5 | 203 ± 4 | 215 ± 6 |
Values are means ± SE.
Top panel: Values for systolic and diastolic BP, n = 12–15/group (2 studies combined); for LV weight and plasma Ang II, n = 8–13/group (preliminary experiment + first study of first experiment).
Lower panel: Values for plasma Ang II and for LV weight at the end of experiment, and for systolic and diastolic BP at baseline (days -2-0) and last 3 days of treatments for vehicles (Veh), combined eplerenone and spironolactone (MR inh), benzamil (Ben) and losartan (Los).
BP was measured in the first experiment (top panel) by intra-arterial catheter after 2 weeks of treatment, and in the second experiment (bottom panel) by continuous telemetry recording. BP responses to the different icv vehicles and to the 2 MR blockers are comparable, and the data for these are shown as the combined data.
P < 0.05 vs. others.
P < 0.05 vs. regular salt.
P < 0.05 vs. baseline.