Figure 4.

SS-induced eNOS activation in blood and plasma perfused vessels. (A) Confocal images show SS-dependent phosphorylation of eNOS-Ser¹¹⁷⁷ in both blood and plasma-perfused vessels. (B) SS-dependent dephosphorylation of eNOS-Thr⁴⁹⁵ occurred only in blood perfused vessels. (C) Negative control of second antibody alone (green, top) and overlay with labelled nuclei (DRAQ5, red, lower image). (D and E) Summary of the fluorescence quantifications (n = 3 or 4 per group). ***P < 0.0005, **P ≤ 0.005, *P < 0.05, NS: P > 0.05 (One-way ANOVA or nested ANOVA with post-hoc Tukey).