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. 2017 May 4;113(11):1351–1363. doi: 10.1093/cvr/cvx089

Figure 6.

Figure 6

LPA-mediated activation of BMP2 relies on phosphorylation of p65 S536. (A–C) P65 S536A inhibits LPA-mediated rise in BMP2 promoter activity (n = 6) (A) and mineralization (n = 6) (B), while p65 S536E mimics the effect of LPA (n = 6) (C). (D–E) ChIP assays; p65 phosphoS536 binds to BMP2 (D), but not to IL6 (E) promoters in response to LPA (n = 6). (F) LPA induces p65 phosphorylation on S536 (n = 4). (G) Y27632 blocks LPA-induced p65 S536 phosphorylation (n = 4). (H) Representative western blot showing that p65 phosphoS536 is increased in CAVS (n = 12) vs. control (n = 10) tissues. (I) p65 S536 phosphorylation relies on IKKα (n = 4). (J) LPA-mediated IKKα phosphorylation is abrogated by Y27632 (n = 6). Values are mean ± SEM. LPA: 10 µM; * P < 0.05.