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. 2017 Sep 7;113(13):1560–1573. doi: 10.1093/cvr/cvx161

Figure 3.

Figure 3

Su/Hx treatment of mice promotes egress of ZsGreen+ progenitor cells from BM. Confocal fluorescence microscopy image of a frozen femoral section from the Rosa26-ZsGreen reporter mice (A), and from the VE-cadherin-cre; ZsGreen double transgenic mice (B). Original magnification 400×, Green: fluorescence protein ZsGreen labelling of cells of endothelial origin; Blue: DAPI counterstaining of nuclei. Su/Hx treatment of the double transgenic mice promotes egress of ZsGreen+ c-kit+ CD45− progenitor cells from BM. (C) Representative flow cytometry analysis plots of BM cells at Nx W0, Su/Hx W1, Su/Hx W2, and Su/Hx W3 are shown. (D) The percentage of ZsGreen+ CD45+ haematopoietic cells in the BM remained unchanged. (E) The percentage of ZsGreen+ c-kit+ CD45− progenitor cells in the BM decreased in a time dependent manner. For both (D) and (E): Nx W0, n = 8; Su/Hx W1, n = 3; Su/Hx W2, n = 4 and Su/Hx W3, n = 15. Data in (D) and (E) are mean ± SEM. *P < 0.05, ***P < 0.001, unpaired two-tailed t-test.