Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2017 Aug 31;113(14):1776–1788. doi: 10.1093/cvr/cvx175

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author 2017. Published by Oxford University Press on behalf of the European Society of Cardiology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

PMC Copyright notice

Cgnl1 silencing impairs Ve-cadherin adherens junction stabilization. (A) Representative micrographs of fluorescent immunostaining for actin (red) and Ve-cadherin (green) distribution in confluent HUVEC monolayers treated with set 1 siCngl1 versus sisham control. Scale bar represents 10 μm. (B) Quantification analysis of percentage of the Ve-cadherin+ area at cell junctions. Values represent means ± SD. *P < 0.05 versus sisham. Images (right) show inverted version of micrographs used for quantification. Data obtained from four different experiments, with analysis of 12 different micrographs per group per experiment. One-way ANOVA. (C) Representative micrographs of z stack analysis of HUVEC-GFP culture on top of pericyte-RFP, showing the fluorescent signal of Ve-cadherin (shown in red, first column) in confluent HUVEC-GFP + (GFP shown in green, second column) layer treated with siCngl1 versus sisham. DAPI signal in blue. Third column shows composite images of the phalloidin blue + (Alexa Fluor 350) pericyte layer beneath the HUVECs-GFP (green). Last column shows isolated Z-stack layer of phalloidin blue+ pericytes (asterisk indicated). Scale bar represents 20 μm. (D) Quantification analysis of percentage of the Ve-cadherin+ area at cell junctions. Values represent means ± SD. *P < 0.05 versus sisham and control. Images (right) show high details of Ve-cadherin at adherens junctions. Scale bar represents 5 μm. Data obtained from four different experiments, with analysis of six different micrographs per group per experiment. One-way ANOVA.