Figure 1.

Deletion of ROCK2 and decreased ROCK activity in platelets from mice with ROCK2 deficiency. (A) Expression of ROCK1 and ROCK2 in platelets from PF4-Cre, ROCK2^fl°^x/fl°^x, ROCK2^Plt−/− mice (n = 5). (B) ROCK expression in heart, bone marrow (BM), monocyte, and platelet of ROCK2^Plt−/− mice (n = 5), (C) ROCK1 and ROCK2 mRNA expression in platelets normalized with GAPDH (n = 3), (D) ROCK activity as determined as a relative blot density ratio of Thr⁸⁵³ phosphorylation of myosin phosphatase target 1 (p-MYPT1) to total myosin phosphatase target 1 (t-MYPT1). β-actin expression served as loading control (n = 5). (E) Representative transmission electron microscopy images of resting and thrombin-stimulated platelets from control (PF4-Cre) and ROCK2^Plt−/− mice (Scale bar, 1 μm. n = 5) used to quantified (F) platelets area (n = 100) and (G) number of pseudopodia per cell (n = 40). *P<0.05, ***P<0.001, ****P<0.00001. All data are expressed as mean±SEM.