Figure 2.

ROCK2 deficiency leads to decreased platelet function. (A) Amount of F-actin as determined by ratio of binding to TRICT-P in response to thrombin in platelets from control (PF4-Cre) and ROCK2^Plt−/− mice. (B) Representative confocal microscopic images of phalloidin-FITC in resting and thrombin-stimulated platelets from PF4-Cre and ROCK2^Plt−/− mice. Scale bar, 5 μm. (C) Blood cell count in PF4-Cre and ROCK2^Plt−/− mice (n = 5). (D) Percent expression of αIIbβ3 and CD62p in response to thrombin in platelets from control (PF4-Cre) and ROCK2^Plt−/− mice. n = 5, *P < 0.05, **P < 0.01. All data are expressed as mean ± SEM.