Figure 3.

Decreased aggregation and adhesion of ROCK2 deficient platelets with prolonged bleeding time and vascular occlusion. (A) Heterotypic and (B) homotypic aggregation in resting and in response to TRAP in platelets from PF4-Cre and ROCK2^Plt−/− mice (n = 5). (C) Confocal microscopy images and quantification of adhesion area (μm²) in resting and TRAP-activated platelets from PF4-Cre and ROCK2^Plt−/−mice (n = 3), (D) Bleeding times of PF4-Cre and ROCK2^Plt−/− mice after tail snipping in saline at 37 °C (n = 20), (E) relative changes in common carotid artery blood flow after application of 5% FeCl3 in PF4-Cre and ROCK2^Plt−/−mice. Blood flow was monitored continuously from time 0 for up to 60 min (n = 8). *P < 0.05, **P < 0.01, ***P < 0.001. All data are expressed as mean±SEM.