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. 2017 Sep 4;113(14):1719–1731. doi: 10.1093/cvr/cvx179

Figure 6.

Figure 6

Confirmation that non-NO-producing platelets initiate adhesion and aggregation reactions while NO-producing platelets form the bulk of an aggregate and limit its size. (A) Merged brightfield-fluorescence microscopy frames from Supplementary material online, video 1 of DAF-FM-loaded human platelets adhering to collagen-coated coverslips. White arrows indicate low/no-NO-producing platelets; black arrows indicate NO-producing platelets. Bars indicate 10 μm. (B) (i and ii) Summary data of low/none-NO-producing and NO-producing platelets binding to collagen over time from N = 3 independent experiments. *P < 0.05.