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. 2018 Jan 24;9(2):59. doi: 10.3390/genes9020059

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Phenotype of Δsnf1. (a) Difference in pigmentation and growth rate between the wild type (WT) or the complement strain snf1-C and Δsnf1. Strains were grown either on PDA or in PDB; (b) The number of conidia produced by NK17, Δsnf1 and the complement strain snf1-C was determined as 5.74 ± 0.35 × 10⁶ (p < 0.01), 0.87 ± 0.15 × 10⁶ (p < 0.01) and 5.74 ± 0.35 × 10⁶ (p < 0.01), per plate, respectively. Each strain was incubated at 28 °C for 168 h. Error bars represent standard deviation; (c) A growth curve based on the quantity of dry-weight of mycelia for NK17, Δsnf1 and the complementation strain snf1-C in PDB at the indicated time points. Dry weight was obtained as the mean value of three parallel cultures. Fungal strains were incubated at 28 °C.