Figure 6.
High performance liquid chromatography (HPLC) profiling for secondary metabolites in Δsnf1. (a) HPLC profiling of the secondary metabolites in Δsnf1 cultured in PDB. The peak patterns including pestalotiollide B peak of Δsnf1 was distinct from that of the wild type; (b) HPLC profiling of the secondary metabolites in Δsnf1 in galactose as sole carbon source. HPLC profiling analysis was repeated for three times; (c) The ratio of peak area of PB peak relative to the dry weight of mycelium was analyzed. The relative yield of PB in Δsnf1 significantly decreased comparing with wild-type, especially in the medium of galactose. Error bars represent standard deviations.