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. 2018 Jan 25;9(2):62. doi: 10.3390/genes9020062

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Phylogenetic tree of fungal lipases. The tree was constructed with 35 characterized fungal lipases (accessions correspond to GenBank unless another source is specified) and 15 putative triacylglycerol lipases from T. harzianum. (*) after the accession numbers are lipases which have been characterized; unlabeled proteins correspond to hypothetical, uncharacterized, predicted lipases. The tree was generated by MAFFT software using the neighbor-joining method [55] with 500 bootstrap re-samplings. Clusters IA, IB and II as described by Yadav et al. [6]. Highlighted in purple letters, the Rhizomocur miehei lipase-like group and in olive green letters, the Candida rugosa lipase-like group, according Schmidt-Dannert [44]. Clade I and Clade II, are consistent with Gupta et al. [76]. T. harzianum triacylglycerol lipases from this study, highlighted in bold red letters.