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. 2018 Jan 29;9(2):66. doi: 10.3390/genes9020066

Figure 1.

Figure 1

Wnt/β-catenin signaling positively regulates in vitro hematopoietic progenitor development. (A) Schematic of differentiation protocol; (B) Reverse transcription quantitative PCR (RT-quantitative PCR) analysis of mesoderm, endothelial, and hematopoietic marker gene expression throughout the differentiation protocol; (C) Schematic of C59 treatment windows and analysis of RUNX1c: Green fluorescent protein (GFP) reporter expression by flow cytometry at d14; (D) RUNX1c:GFP+ cells normalized to control (0 nM C59, 0.1% dimethyl sulfoxide (DMSO) treated) cells. Tx = treatment; (E) %RUNX1c:GFP+ (green dot) and %CD34+/CD45+ (black square) cells in C59 or control (0 nM C59, 0.1% DMSO treated) cells; (F) Schematic of CHIR98014 (CHIR) treatment and analysis of RUNX1c:GFP reporter expression by flow cytometry at d14; (G) RUNX1c:GFP+ cells normalized to control (0 nM CHIR, 0.1% DMSO treated) cells; (H) RUNX1c:GFP+ cells normalized to control (0 nM CHIR, 0.1% DMSO treated) cells, treated with the indicated dose of CHIR added at days 2–4. Data shown are representative of two biological replicates. Error bars represent standard deviation; * = p value < 0.05, ** = p value < 0.01, *** = p value < 0.001, n.s. = Not significant.