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. 2018 Jan 29;9(2):66. doi: 10.3390/genes9020066

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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WNT9A has a dose-dependent effect on hematopoietic differentiation efficiency. (A) Schematic of doxycycline-inducible WNT9A transgene. (B) Schematic of doxycycline induction of WNT9A expression and flow cytometry readout for the data shown in (D,E). (C) Immunoblot (IB) showing increasing WNT9A protein expression in response to the dosages of doxycycline shown. Top: WNT9A, Bottom: β-ACTIN loading control. (D) Representative flow cytometry plots for data graphed in (E); (E) Percentage of CD34⁺/CD45⁺ cells normalized to untreated control. Data represent the average of three biological replicates. ** = p value < 0.01, *** = p value < 0.001, n.s. = Not significant. Error bars represent standard deviation.