Official websites use .gov
A
.gov website belongs to an official
government organization in the United States.
Secure .gov websites use HTTPS
A lock (
) or https:// means you've safely
connected to the .gov website. Share sensitive
information only on official, secure websites.
Construction of the pUPH plasmid. Primers XhoI-6MPK_Up and SmaI-6MPK_Do were used to amplify TK0664 from the T. kodakarensis KOD1 genome. Then, TK0664 and the vector pUFH were digested by SmaI (S) and XhoI (X) and ligated after a gel purification step, to form plasmid pUPH where pyrF is replaced by TK0664. The restriction sites BamHI (B) and KpnI (K) were conserved to enable cloning of the homologous regions in pUPH.
