Table 2.
List of primers used in this study.
| Primers Used for Amplification of Flanking Regions of Targeted Gene | Sequence (5′-3′) | Tm (°C) |
|---|---|---|
| PolB_1Up | AAAAAAGGTACCGCTTAACATTCCTGACTCCCAGAATCTT | 59.4 |
| PolB_1Do | TCTATTTCATTAAATCACCTAATTTCACCCTTTTAAAAATACATGCCCAT | 57.9 |
| PolB_2Up | ATTTTTAAAAGGGTGAAATTAGGTGATTTAATGAAATAGAATGAGCAGGA | 57.9 |
| PolB_2Do | AAAAAAGGATCCCGGCTTCTGGGGAAACCTCG | 60.6 |
| 6MPb_1Up | AAAAAACGTACCAAGAAAACCGGAGTTTTAGTGAATACACC | 58.4 |
| 6MPb_1Do | TCTCATGGAAACATTTAAATGGTTGTGGTATCTTGGACAAGAAGAAAA | 59.5 |
| 6MPb_2Up | TTGTCCAAGATACCACAACCATTTAAATGTTTCCATGAGAAAAATGAAATGCAAAAA | 60.3 |
| 6MPb_2Do | AAAAAAAGATCTCTCGCTCTAAAGGAGCTTTCAACA | 56.0 |
| RHII_1Up | AAAAAAGGTACCCGGTACCCTGATAAAGAAGGCATC | 59.4 |
| RHII_1Do | TTAGTATTCAGGAAATGAGGACTCTTGAGGTTCTTTCTCTTCGGT | 61.3 |
| RHII_2_Up | AGAGAAAGAACCTCAAGAGTCCTCATTTCCTGAATACTAACGTTCCG | 63.0 |
| RHII_2_Do | AAAAAAAGATCTACGACTCATGATGTTAATCCTCTTACAGAG | 57.4 |
| Primers Used to Check Mutants | Sequence (5′-3′) | Tm (°C) |
| PolB_Up | GTCAGCTATGAGCTCGTGAAGAGTTAT | 59 |
| PolB_Do | AGAAGAGCGTAAATGTAAGGCTGGA | 59 |
| 6MPk_Up | AAAAAACTCGAGCCCGTCCAAGCTACCACTCCC | 69 |
| 6MPk_Do | AAAAAACCCGGCTTCAAAACCCAGCCAAACAACACCC | 71 |
| 6MPb_Up | ACCTCAGACATCTCTGCTGCTTG | 60 |
| 6MPb_Do | GCCCCGATAAGTGCTGAAAGATACA | 60 |
| RHII_Up | GAGGAATCGCTGAAGTTTTACTGGG | 59 |
| RHII_Do | CTACAAAGAGCATGGCGAATTTCCG | 60 |
Tm: Melting temperature.