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. 2018 Feb 13;9(2):86. doi: 10.3390/genes9020086

Figure 3.

Figure 3

Splicing analysis of TTN Novex 2 between species. (A) PCR products with primer pairs spanning exon 42 and 46 (1) and exon 46 and 49 (2); human, chicken, and frog express Exons 42–46 and Exons 46–49 with a lower level; no products could be detected in pig, mouse, or rat. (B) Schematic diagram of the reported Novex 2 splicing pattern in human, chicken, and frog heart. (C) Schematic diagram of PCR results; a truncated form with Intron 46 retention was found in human and frog heart. (D) Schematic diagram of PCR results; a new truncated form with co-expression of exons 45 and 46 and Intron 46 retention was observed in chicken and frog hearts. (E) PCR products with primer spanning exon 45 and Intron 46 between species; DNA band was observed in pig, mouse, and rat heart. (F) Schematic diagram of a newly identified truncated form with co-expression of Exons 45 and 46 and Introns 45 and 46 retention in pig, mouse, rat, and chicken. M: DNA marker; E: Exon; black boxes: Exons; white boxes: Intron retention; black lines: Introns.