Splicing pattern and expression level of Novex isoforms in human cardiomyopathies. (A) PCR products with primers spanning exons 44–45 (Novex 1), exons 44–46 (Novex 2), and exons 44–48 (Novex 3) in normal and diseased hearts; no splicing pattern difference was found between normal and diseased hearts, but expression levels of Novex isoforms was observed. (B) Quantification of DNA band intensity of Novex 1 in HCM, DCM, and ARVC. (C) Quantification of DNA band intensity of Novex 2 in HCM, DCM, and ARVC. (D) Quantification of DNA band intensity of Novex 3 in HCM, DCM, and ARVC; Gapdh: internal control; HCM: hypertrophy cardiomyopathy; DCM: Dilated cardiomyopathy; ARVC: Arrhythmogenic right ventricular cardiomyopathy. Mean ± (Standard Error of the Mean) SEM, ** p < 0.001; ####
p < 0.00001.