Skip to main content
. 2018 Feb 14;9(2):96. doi: 10.3390/genes9020096

Figure 3.

Figure 3

Summary of an integrative cytogenomic study on the rDNAome in European pikes (Esox lucius and E. cisalpinus). (A) Fluorescence in situ hybridization (FISH) with 5S rDNA (red) and 45S rDNA (green); (B) Distribution of single-nucleotide polymorphism (SNPs) along the E. lucius 5S rDNA unit obtained from Illumina reads showing absence of SNPs in the internal controlling region composed of Box-A, IE and Box-C elements; (C) Distribution of variants in intergenic spacer regions (IGS) in three PacBio reads (a–c). Slanted lines indicate tandemly arranged units visualized through the alignment of reads (x-axis) with a 5S gene (y-axis); (D) Higher-order organization of 5S rDNA arrays in E. lucius. Self-to-self comparison of long PacBio molecules representing three groups; (E) 5S rRNA domain reconstruction of E. lucius indicating its potential functionality; (F) Methylation analysis of 5S rDNA by the methylation-sensitive HpaII (H) restriction enzyme and its methylation-insensitive MspI (M) isoschizomere. Reproduced from [8].