Table 2.
Evaluation of TFSSR primer pairs for the different repeat classes (based on 8 genotypes).
| Parameters | Di | Tri | Tetra | Penta | Hexa | Total/Average |
|---|---|---|---|---|---|---|
| Tested primer | 20 | 47 | na | 1 | 3 | 71 |
| PCR amplification | 18 | 47 | na | 1 | 3 | 69 |
| Band Specific | 9 | 28 | na | 0 | 2 | 39 |
| Scorable Primer | 11 | 31 | na | 0 | 2 | 44 |
| Polymorphic | 10 | 29 | na | 0 | 2 | 41 |
| Range of Alleles No. | 2–5 | 2–6 | na | na | 2–3 | 2–6 |
| Total No. of Alleles | 50 | 146 | na | 4 | 7 | 207 |
| No. of Homozygous | 12 | 40 | na | 1 | 2 | 55 |
| No. of Heterozygous | 6 | 7 | na | 0 | 1 | 14 |
| Homo:Hetero Ratio | 2:1 | 6:1 | na | na | 2:1 | 4:1 |
| Mean of Alleles ±SD | 2.78 ± 0.94 | 3.11 ± 1.5 | na | na | 2.33 ± 0.58 | 3.06 ± 0.755 |
| PIC±SD | 0.55 ± 0.17 | 0.64 ± 0.15 | na | 0.47 ± 0 | 0.65 ± 0.16 | 0.58 ± 0.12 |
PIC: Polymorphic Information Content; SD: Standard Deviation; na: not available.