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. 2017 Jan 17;113(3):343–353. doi: 10.1093/cvr/cvx005

Figure 6.

Figure 6

Reversal of RyR oxidation in TAB myocytes by SK-enhancers via attenuation of mitochondrial ROS. (A) CyPPA reduced thiol-oxidation of RyR in TAB ventricular myocytes. Cells were incubated with 10 μmol/L CyPPA for 10 min before lysis. ROS scavenger dithiothreitol (10 mmol/L) and oxidant 2,2′-dithiodipyridine (200 μmol/L) were used to obtain minimal and maximal oxidation for normalization. Top: Representative fluorescence images of oxidation-sensing dye mBB. Bottom: RyR western for normalization. (B) Pooled data for A. Additional group of cells was treated with 10 μmol/L NS309 for 10 min in the same way as CyPPA, *P < 0.05 vs. Sham and #P < 0.05 vs. TAB, n = 4 hearts for both Sham and TAB, one-way ANOVA. (C) SK modulators alter rate of ROS production measured with MitoSOX in CMs. Cell numbers are shown in the columns. *P < 0.05 vs. Sham and #P < 0.05 vs. TAB, hierarchical linear model. Number of Sham animals was 3; number of Tab animals was 4.