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. 2017 Jul 20;113(14):1753–1762. doi: 10.1093/cvr/cvx115

Figure 2.

Figure 2

Mmp2 gene deletion reduced Ang II-induced reactive oxygen species (ROS) generation and extracellular matrix remodelling. ROS generation by dihydroethidium (DHE) staining in the aortic media and adventitia and perivascular adipose tissue (PVAT) (A and D), media fibronectin expression by immunofluorescence (B and E) and adventitial collagen content by Sirius red staining (C and F) were determined in the same groups as in Figure 1. Representative images of DHE staining (A), fibronectin (B) immunofluorescence images and RGB thresholded images of Sirius red staining (C) of aortic sections are shown. Green fluorescence in A and B represents elastin autofluorescence. Values are means ± SEM. Number of samples per group for media in D: Mmp2-/- = 7 and other groups = 6. For Adventitia + PVAT in D: Mmp2-/- = 5 and other groups = 6. For E: controls = 5 and Ang II-treated groups = 6. For F: Mmp2-/-+Ang II = 6 and other groups = 5. Data were analysed using two-way ANOVA followed by a Student–Newman–Keuls post hoc test. *P < 0.05 and **P < 0.001 vs. their respective controls and †P < 0.001 vs. WT + Ang II.