Figure 4.

Hypoxia causes ROCK-dependent translocation of TRPC6 and PTEN to caveolae. Caveolar fractions were isolated from PASMC (input: whole cell lysate) by sucrose gradient centrifugation, and identified by the presence of the marker protein caveolin-1. Representative immunoblots show (A) absence of PTEN and TRPC6 from caveolae of normoxic PASMC, but (B) recruitment to caveolar fractions within 15 min of hypoxia (1% O₂), that was (C) blocked by Y27632 (5 µMol/L). (D) Group data from n = 3–5 independent experiments each show caveolar recruitment of TRPC6 and PTEN, expressed as fraction of protein detected in caveolar fractions. Group data are means ± SEM, *P < 0.05 vs. normoxia.