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. 2017 Feb 2;113(3):310–320. doi: 10.1093/cvr/cvx004

Figure 5.

Figure 5

The JAK2 inhibitor AG490 and PDGF-receptor inhibitor AG1296 reverse PDGF-AB action in canine atrial fibroblasts. (A) Representative immunoblots of p-STAT3 and t-STAT3 in fibroblasts stimulated with PDGF-AB with or without AG490 (20 µM) or AG1296 (10 µM). (B–D) Quantitative protein-expression of p-STAT3, t-STAT3, and p-STAT3/t-STAT3 ratio. (E) Representative immunoblots of secreted collagen-1 from culture media from LA fibroblasts stimulated with PDGF-AB with or without AG490 (20 µM) or AG1296 (10 µM). (F) Fibroblast cell-count (cells/culture-dish) after 48-hour incubation with PDGF-AB with or without AG490 (20 µM) or AG1296 (10 µM). (G) Band-intensities for secreted collagen-1 (normalized to cell number). All values were normalized to controls (without vehicle) on the same blot. Mean ± S.E.M., *P < 0.05, **P < 0.01, ***P < 0.001. n = 8–14/group. One-way ANOVA with Bonferroni-corrected t-tests were used for statistical analysis. COL-1, collagen type I; Veh, vehicle; P, PDGF.