Figure EV5. Impact of EL86 (guide strand) 2′‐MOE modifications on the knockdown efficiency of endogenous ELAVL1 mRNA in HeLa cells.

Remaining mRNA level (y‐axis) was quantified by RT–qPCR (mean ± SD from two replicates). The positions of the modified residues are shown on the x‐axis. “wt” is the mRNA level quantified for unmodified EL86. Contacts between dsRBD12 and EL86 observed in our NMR structure (complex A) are shown with white (black) stars for those involving dsRBD1 (dsRBD2).