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. 2018 Mar 14;38(11):2863–2876. doi: 10.1523/JNEUROSCI.2362-17.2018

Figure 4.

Figure 4.

Induction of rapid homeostatic synaptic potentiation in hippocampal neurons increases phosphorylation of GluA1–S845 by AKAP150-anchored PKA in opposition to AKAP150-anchored CaN. A, Representative immunoblots of whole-cell extracts of hippocampal neurons cultured from WT, AKAP150 KO, ΔPKA, or ΔPIX mice to detect phospho-GluA1-S845 (pS845) levels followed by stripping and re-probing to detect total GluA1 levels. Note: Left, Extracts from WT and KO were analyzed on the same blot using the same exposures, but then the lanes were rearranged for presentation. B, Quantification of the ratio of pS845/total GluA1 normalized to WT control (always present on the same blot) shows that induction of rapid homeostatic scaling-up with 4 h TTX/MK significantly increases S845 phosphorylation in neurons from WT (*p < 0.05 by unpaired t test) but not AKAP150 KO or ΔPKA mice. Neurons from AKAP150ΔPIX mice already exhibit increased basal S845 phosphorylation and do not show any further increase after 4 h TTX/MK treatment (#p < 0.05 to WT Control by unpaired t test). Error bars indicate SEM.