Table 2.
Changes in body weight, food intake, cecal H2, organic acid production, and cecal counts of total anaerobes in HAS-fed rats transplanted with high H2-producing microbiota.
| Control | Transplantation | p Value | |
|---|---|---|---|
| Initial body weight (g on day 0) | 238 ± 2 | 237 ± 2 | 0.9753 |
| Body weight gain (g/13 day) | 87 ± 4 | 78 ± 4 | 0.1348 |
| Food intake (g/13 day) | 287 ± 7 | 261 ± 8 * | 0.0493 |
| Net H2 excretion (μmol/5 min) | |||
| Day 0 | 0.103 (0.064–0.338) | 0.146 (0.101–0.176) | 0.8931 |
| Day 3 | 0.579 (0.447–1.13) | 0.405 (0.196–0.830) | 0.1690 |
| Day 6 | 0.334 (0.212–0.679) | 0.304 (0.151–0.622) | 0.8786 |
| Day 10 | 0.554 (0.199–0.878) | 1.13 (0.252–2.92) | 0.1734 |
| Day 13 | 0.721 (0.347–3.55) | 2.34 (1.65–2.96) | 0.3910 |
| AUCday 6–13 (mmol) | 0.966 (0.665–2.64) | 3.04 (1.41–6.06) | 0.1627 |
| Portal H2 (μmol/L) | 3.07 ± 1.00 | 9.95 ± 1.78 ** | 0.0041 |
| Cecum (g) | |||
| Contents | 9.31 ± 0.58 | 9.59 ± 0.61 | 0.7443 |
| Tissue | 1.87 ± 0.11 | 1.81 ± 0.10 | 0.7253 |
| Cecal organic acids (μmol/g) | |||
| Acetate | 43.5 ± 3.3 | 57.8 ± 2.7 ** | 0.0038 |
| Propionate | 9.80 ± 0.98 | 8.17 ± 1.07 | 0.2777 |
| n-Butyrate | 5.65 ± 1.05 | 7.06 ± 0.80 | 0.2999 |
| Succinate | 61.4 ± 4.2 | 48.5 ± 5.2 | 0.0701 |
| Cecal bacteria | |||
| Total anaerobes (log10cfu/g) | 12.7 ± 0.5 | 12.6 ± 0.3 | 0.7592 |
Data are expressed as the means ± standard error (SE) or medians (25%–75%), control group, n = 10 and transplantation groups, n = 11. Data were analyed using the Student’s t-test (data expect for net H2 excretion and area under the curve (AUC)) or Welch’s test (data for net H2 excretion and AUC). Transplantation of the inoculum was performed on days 3 and 4. Net H2 excretion in breath and flatus was measured. AUC was calculated from changes in net H2 excretion from day 6 to day 13. *, ** Mean values were significantly different from those of the control group (p < 0.05 and p < 0.01, respectively).