. 2018 Jan 31;10(2):163. doi: 10.3390/nu10020163

Table 1.

Specific primers used for real-time quantitative PCR.

Gene	Primer	Product Size (bp)	Annealing Temperature (C)
GAPDH	F: AAGCTCATTTCCTGGTATGACAACG	126	61.3
GAPDH	R: TCTTCCTCTTGTGCTCTTGCTGG	126	61.3
PPAR-γ	F: ATGACAGACCTCAGACAGATTG	210	54
PPAR-γ	R: AATGTTGGCAGTGGCTCAG	210	54
LDLR	F: ACTTACGGACAGACAGACAG	223	57
LDLR	R: GGCCACACATCCCATGATTC	223	57
IL-1	F: GCTTCTCTCTGGTCCTTGG	174	56
IL-1	R: AGGGCAGGGTAGAGAAGAG	174	56
IL-8	F: GCAGAGGGTTGTGGAGAAGT	150	56
IL-8	R: ACCCTACAACAGACCCACAC	150	56
TNF-α	F: GTCAACCTCCTCTCTGCCAT	188	52
TNF-α	R: CCAAAGTAGACCTGCCCAGA	188	52

GAPDH, glyceraldehyde-3-Phosphate dehydrogenase; IL-1, interleukin-1; IL-8, interleukin-8; LDLR, low-density lipoprotein receptor; PPAR-γ, peroxisome proliferator-activated receptor gamma; TNF-α, tumor necrosis factor alpha.