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. 2018 Jan 31;10(2):165. doi: 10.3390/nu10020165

Figure 3.

Figure 3

(A) Muscle mRNA expression of Pgc1α, Creb1, Ampka1, Ampka2, Mkp1, Slc2a4, Nrf1, Nrf2, Sirt1 and Mttfa after eight weeks of treatment; (B) mtDNA quantification after eight weeks of treatment in gastrocnemius muscle; (C,D) Muscle protein level of PGC1α and p-Ampk/Ampk and illustrative images of protein content by western blotting; (E,F) Muscle protein level of subunits of the five OXPHOS complexes and illustrative images of protein content by western blotting. All samples were normalized using housekeeping 18S gene and, afterwards, the HFD-GUA group was normalized by the HFD group. The equation 2−ΔΔCt was used to calculate the fold change. * p < 0.05 using Student’s t-test. Black bars correspond to the HFD group (n = 6) and grey bars correspond to the HFD-GUA group (n = 6). Error bars reflect SEM.