Table 2.
Primers used for real-time PCR.
| Gene | Primer | Sequence (5′→3′) |
|---|---|---|
| Sirt1 | Sense | AGTGGCACATGCCAGAGTC |
| Antisense | TCCAGATCCTCCAGCACAAT | |
| Creb1 | Sense | TTTGTCCTTGCTTTCCGAAT |
| Antisense | CACTTTGGCTGGACATCTTG | |
| Ampka1 | Sense | TGAGAACGTCCTGCTTGAATG |
| Antisense | ATCATTGGCTGAGCCACAGC | |
| Ampka2 | Sense | ACAGGCCATAAAGTGGCAGT |
| Antisense | GTCGGAGTGCTGATCACGTG | |
| Pgc1α | Sense | CCGAGAATTCATGGAGCAAT |
| Antisense | TTTCTGTGGGTTTGGTGTGA | |
| Nrf1 | Sense | CAACAGGGAAGAAACGGAAA |
| Antisense | CACTCGCGTCGTGTACTCAT | |
| Nrf2 | Sense | AGGACATGGAGCAAGTTTGG |
| Antisense | TCTGTCAGTGTGGCTTCTGG | |
| Ucp1 | Sense | TCAGGGCTGAGTCCTTTTGT |
| Antisense | CTGAAACTCCGGCTGAGAAG | |
| Ucp3 | Sense | CTCACTTTTCCCCTGGACAC |
| Antisense | GTCAGGATGGTACCCAGCAC | |
| 18S | Sense | AAACGGCTACCACATCCAAG |
| Antisense | CAATTACAGGGCCTCGAAAG |