Table 1.
Baseline laboratory measurements in the 2015 ISA-Capital. São Paulo, 2015.
| Analyte | Sample | Technical Reference |
|---|---|---|
| Vitamin A (retinol) | Heparinized plasma | High performance liquid chromatography method (Ref. # KC 1600; Immundiagnostik AG, Bensheim, HE, Germany) |
| Vitamin C (ascorbic acid) | Heparinized plasma | High performance liquid chromatography method (Ref. # KC 2900; Immundiagnostik AG, Bensheim, HE, Germany) |
| Vitamin E (alpha-tocopherol) | Heparinized plasma | High performance liquid chromatography method (Ref. # KC 1600; Immundiagnostik AG, Bensheim, HE, Germany) |
| Vitamin D (cholecalciferol-25 (OH)D3) | Serum | Chemiluminescence (Diasorin kit, Stillwater, MN, USA) |
| Vitamin B6 (pyridoxal-5-phosphate) | Heparinized plasma | High performance liquid chromatography method (Ref. # KC 2100; Immundiagnostik AG, Bensheim, HE, Germany) |
| Vitamin B9 (folate) | Serum and erythrocytes | Chemiluminescent immunoassay (Ref. # A98032; Beckman Coulter Inc.; Fullerton, CA, USA) |
| Vitamin B12 (cobalamin) | Serum | Chemiluminescent immunoassay (Ref. # 3300; Beckman Coulter Inc.; Fullerton, CA, USA) |
| Parathormone | Serum | Elecsys 2010 kit, Roche Diagnostics, Indianapolis, ID, USA |
| Iron | Serum | Colorimetric assay (Cobas; Roche Diagnostics GmbH, Mannheim, BW, Germany) |
| Ferritin | Serum | Chemiluminescent immunoassay (Ref. # 33020; Beckman Coulter Inc.; Fullerton, CA, USA) |
| Transferrin | Serum | Kinetic nephelometry (Ref. # OSR6152; Beckman Coulter Inc.; Fullerton, CA, USA) |
| Whole blood cell count | Whole blood | Automatic method |
| Magnesium | Serum | Photometric color assay (Ref. # OSR6189; Beckman Coulter Inc.; Fullerton, CA, USA) |
| Zinc | Serum | Flame atomic absorption method (AAnalyst 100 instrument; Perkin Elmer, Norwalk, CT, USA) [10] |
| Total cholesterol | Serum | Trinder reaction (cholesterol oxidase) (Cobas; Roche Diagnostics GmbH, Mannheim, BW, Germany) |
| Low-density lipoprotein-cholesterol | Serum | Homogeneous enzymatic colorimetric assay (Cobas; Roche Diagnostics GmbH, Mannheim, BW, Germany) |
| High-density lipoprotein-cholesterol | Serum | Homogeneous enzymatic colorimetric assay (Cobas; Roche Diagnostics GmbH, Mannheim, BW, Germany) |
| Triacylglycerol | Serum | Enzymatic colorimetric assay (glycerol phosphate peroxidase) (Cobas; Roche Diagnostics GmbH, Mannheim, BW, Germany) |
| Very low-density lipoprotein-cholesterol | Serum | Values were calculated by dividing the triglyceride values by five |
| Non-high-density lipoprotein-cholesterol | Serum | Values were determined as the difference between the values of total cholesterol and high-density lipoprotein-cholesterol |
| Glucose | Sodium fluoride plasma | Colorimetric enzymatic assay of glucose oxidase (Trinder reaction) (Cobas; Roche Diagnostics GmbH, Mannheim, BW, Germany) |
| Insulin | Ethylenediamine tetraacetic plasma | Multiplex immunoassay (LINCOplex®; Linco Research Inc., St. Charles, MO, USA) |