Figure 4.

Plant miRNA detection after in vitro digestion. AIN-93M diet supplemented with 3% fresh corn powder, corn miRNA extract, or methylated or unmethylated miR171j were treated with in vitro digestion buffer as described in the Materials and Methods. Recovery was calculated as the percentage of miR167a or miR171j detected after each phase of digestion comparing to the amount measured in the starting materials. All treated samples are significantly different from the respective controls at p < 0.05.