Table 3.
Effect of OA on bone mineral density and bone microarchitecture at proximal tibia, distal femur, and lumbar vertebra in OVX mice analyzed by micro-CT.
| Sham | OVX | E2 | OAL | OAH | |
|---|---|---|---|---|---|
| Proximal tibia | |||||
| BMD, mg HA/cm3 | 207.2 ± 7.7 | 116.7 ± 6.5 ^^^ | 249.4 ± 7.5 *** | 170.5 ± 6.8 *** | 175.4 ± 4.7 *** |
| BV/TV, % | 23.3 ± 1.1 | 11.5 ± 1.0 ^^^ | 27.9 ± 1.4 *** | 18.0 ± 0.8 *** | 19.0 ± 0.7 *** |
| Tb.N, mm−1 | 4.51 ± 0.24 | 3.08 ± 0.37 | 5.38 ± 0.29 *** | 3.76 ± 0.15 | 3.74 ± 0.08 |
| Tb.Th, μm | 51.2 ± 0.8 | 42.9 ± 1.6 ^^^ | 54.0 ± 1.2 *** | 48.7 ± 0.8 ** | 50.7 ± 1.1 *** |
| Tb.Sp, μm | 175.4 ± 11.2 | 312.2 ± 34.7 ^^^ | 126.5 ± 17.3 *** | 225.4 ± 11.1 * | 217.6 ± 6.5 * |
| Conn.D, mm3 | 196.1 ± 15.9 | 105.6 ± 8.3 ^^ | 237.2 ± 27.7 *** | 159.6 ± 10.0 *** | 140.4 ± 8.9 * |
| Distal femur | |||||
| BMD, mg HA/cm3 | 198.4 ± 6.6 | 130.9 ± 9.1 ^^^ | 246.8 ± 8.4 *** | 185.9 ± 4.3 *** | 195.6 ± 7.2 *** |
| BV/TV, % | 16.6 ± 0.9 | 12.0 ± 1.0 ^^ | 25.5 ± 1.3 *** | 18.5 ± 0.8 *** | 20.7 ± 1.1 *** |
| Tb.N, mm−1 | 3.50 ± 0.08 | 2.79 ± 0.18 ^ | 4.24 ± 0.18 *** | 3.57 ± 0.14 * | 3.81 ± 0.21 *** |
| Tb.Th, μm | 50.3 ± 1.5 | 41.5 ± 1.4 ^^^ | 59.4 ± 2.1 *** | 51.9 ± 1.4 *** | 54.2 ± 0.4 *** |
| Tb.Sp, μm | 239.7 ± 8.0 | 329.8 ± 27.6 ^^ | 176.3 ± 11.0 *** | 231.6 ± 10.9 ** | 214.2 ± 15.9 *** |
| Conn.D, mm3 | 141.8 ± 11.5 | 97.4 ± 7.9 ^ | 141.6 ± 10.8 * | 144.5 ± 8.5 * | 149.2 ± 10.7 ** |
| Lumbar vertebra | |||||
| BMD, mg HA/cm3 | 231.8 ± 11.0 | 183.1 ± 7.1 ^^^ | 297.0 ± 15.3 *** | 219.0 ± 8.0 ** | 213.2 ± 4.7 * |
| BV/TV, % | 29.4 ± 1.1 | 21.1 ± 0.6 ^^^ | 37.0 ± 1.7 *** | 25.6 ± 1.3 ** | 25.1 ± 0.8 * |
| Tb.N, mm−1 | 4.62 ± 0.12 | 3.82 ± 0.08 ^^^ | 4.89 ± 0.11 *** | 4.26 ± 0.13 * | 4.33 ± 0.12 ** |
| Tb.Th, μm | 63.5 ± 1.0 | 58.3 ± 1.9 | 75.3 ± 2.0 *** | 59.3 ± 1.5 | 57.0 ± 1.0 |
| Tb.Sp, μm | 153.8 ± 6.3 | 208.8 ± 5.7 ^^^ | 130.0 ± 6.3 *** | 176.1 ± 7.7 ** | 172.9 ± 5.2 ** |
| Conn.D, mm3 | 141.1 ± 7.1 | 100.2 ± 4.9 ^^^ | 139.7 ± 4.3 *** | 140.8 ± 6.5 *** | 140.3 ± 6.6 *** |
Four-month-old ovariectomized (OVX) or sham-operated (Sham) C57BL/6J mice were pair-fed with phytoestrogen-free AIN-93M diet and treated with vehicle (Sham or OVX), E2 (200 μg/kg/day), OA low dose (OAL, 50 mg/kg/day) or OA high dose (OAH, 100 mg/kg/day) for 6 weeks. Bone mineral density (BMD) and bone microarchitecture parameters were measured by microCT: bone volume/total volume (BV/TV), trabecular number (Tb.N), trabecular thickness (Tb.Th), trabecular separation (Tb.Sp), and connectivity density (Conn.D). Data are presented as mean ± SEM and analyzed by one-way ANOVA followed by Tukey’s multiple comparison tests. ^ p < 0.05, ^^ p < 0.01, ^^^ p < 0.001 vs. Sham; and * p < 0.05, ** p < 0.01, *** p < 0.001 vs. OVX.