Fig. 1.

Basal nociception assay. a The Duox (CG3131) locus. The translation start site is marked with an angled arrow. The MiMiC element (Duox [MI11825]) was inserted into the 2nd intron. The UTRs, coding region, and introns are denoted in gray, black, and white, respectively. b RT-PCR of wild-type (w1118) and Duox [MI11825] heterozygous larvae. Binding sites of the primers used for PCR are indicated by short red lines in (a). The ribosomal protein Rp49 was used as a loading control. c Larval thermal nociception assay. Larval nociceptive response was counted if it occurred within 10 s of heat exposure (n = 60 per time section). d Larval mechanical nociception. Larval mechanical response to a mechanical force (30 and 45 mN) was counted (n = 75 per each mechanical force). Error bars denote +/− SEM. One-way ANOVA with Tukey post-test was used to analyze the differences. n.s., non-significant