Fig. 2.

Thermal nociceptive sensitization assay. Larvae were irradiated with UV at 2 mJ/cm². Larval response within 10 s to heat exposure (40 °C) was counted as nociceptive response. a Wild-type larvae (n = 90 per time section). b TrpA1 homozygous mutant larvae (n = 75 per time section). c Duox heterozygous mutant larvae (n = 90 per time section). d Duox silencing in mdIV neurons. Ppk > duox-RNAi denotes ppk-Gal4 > UAS-Duox-RNAi (38907) (n = 60 per time section). e TrpA1 silencing in mdIV neurons. Ppk > TRPA1-RNAi denotes ppk-Gal4 > UAS-TrpA1-RNAi (n = 45 per time section). f Ppk1 silencing in mdIV neurons. Ppk > ppk1-RNAi denotes ppk-Gal4 > UAS-ppk1-RNAi (n = 45 per time section). Error bars denote +/− SEM. One-way ANOVA with Tukey post-test was used to analyze the differences. *, ** and *** indicate p < 0.05, 0.01, and 0.001 respectively. n.s., non-significant