Fig. 3.

Mechanical nociceptive sensitization assay. Larvae were irradiated with UV at 2 mJ/cm² . Larval nociceptive response to mechanical force (30 mN) was evaluated. a Wild-type (n = 120 per time section), TRPA1 homozygous mutant (n = 45 per time section), and duox heterozygous mutant larvae (n = 75 per time section). b Duox silencing in mdIV neurons. Ppk > duox-RNAi indicates ppk-Gal4 > UAS-duox RNAi (n = 60 per time section). c TrpA1 silencing in mdIV neurons. Ppk > TRPA1-RNAi indicates ppk-Gal4 > UAS-TRPA1 RNAi (n = 45 per time section). Error bars denote +/− SEM. One-way ANOVA with Tukey post-test was used to analyze the differences. *, ** and *** indicate p < 0.05, 0.01, and 0.001 respectively. n.s., non-significant