Fig. 6.

Prediction of absolute focus quality on an unseen cell type (MCF-7 cells, from BBBC021 dataset [12]) but familiar stain, Hoechst. An 11 × 10 image montage showing sample patch-level predictions (for each predicted defocus level (0 for in-focus, 10 for most out-of-focus, corresponding to an approximate blur diameter of 30 pixels) and certainty bin (1.0 is most certain); hue and lightness encode predicted defocus level and certainty, respectively. Blank regions denote combinations of predicted defocus level and certainty for which there are no model predictions for this particular dataset. Scale bar is 10 μm or 30 pixels