Fig. 5.

Structural effects of NSC on the gRNA. The first 411 nts of the gRNA, containing the packaging signal region and beginning of the gag ORF were in vitro transcribed and refolded. RNA was treated with NSC in DMSO or DMSO only. Acylation sensitivity at each nucleotide was used to model the structure and predicted free energy. The most stable structure is shown. a Predicted RNA structure and stability of DMSO treated RNA. b Predicted RNA structure and stability of NSC treated RNA. c, d Acylation sensitivity difference upon NSC treatment. c Differences > or < 0 and d differences > or < 0.1 reactivity units