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. 2018 Mar 14;18:43. doi: 10.1186/s12870-018-1246-0

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Comparative analysis on the PM subcompartmentation using LSCM and VAEM. To obtain a detailed view of the membrane domains, LSCM was firstly used to image the upper surface plane for cotyledon epidermal cells; VAEM and kymograph analysis were utilized to capture protein dynamics on the PM. a AtFlot1a displayed obviously uneven localization under LSCM and high lateral motility under VAEM, arrows indicate deviations in kymograph curves; (b-d) 35S:StREM, 35S:AtREM1.2, pREM1.2:AtREM1.2 showed diffused but uniformly distributed fluorescence labeling of the PM, while they formed puncta showing low lateral motility under VAEM; (e-f) pSKU5:SKU5-GFP and pCOBRA:COBRA-GFP demonstrated similar distribution patterns to those of REMs, but displayed relatively higher lateral motility. Bars = 50 μm (LSCM), 10 μm (VAEM); Interval between frames = 200 ms