Fig. 1.

Proliferation of BMSCs in HPGF-C18 and FBS. Cryopreserved passage 2 BMSCs from 3 healthy subjects were thawed and cultured for 2 additional passages in αMEM supplemented with 5, 10, 15 and 20% HPGF-C18 and 20% FBS. The cumulative population doublings (PDs) at passages 3 and 4 are shown in a. The mean (± 1 SD) cumulative PDs normalized by total number of days in culture at passage 4 for each culture condition is show in b. Primary cultures of marrow aspirates cultured in αMEM supplemented with 10% HPGF-C18 showed irregular spacing and piling up of BMSCs while primary cultures of the same marrow aspirated cultured in 20% FBS formed more uniform monolayers (c). BMSCs from marrow aspirates of 5 healthy subjects were cultured for 4 passages in 10% HPGF-C18 and 20% FBS. The cumulative population doubling for BMSCs grown in 10% HPGF-C18 are shown in green and of 20% FBS in red. Each panel represents BMSCs isolated and expanded from one healthy subject. Each circle represents one BMSC passage (d)